Purification and molecular characterization of chitinases from soil actinomycetes
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چکیده
منابع مشابه
Production, purification, characterization and over-expression of xylanases from actinomycetes.
Xylanases are a group of depolymerizing enzymes often used for the hydrolysis of xylan (present in hemicellulose) to monomeric sugars and comprise endo-xylanases (EC 3.2.1.8) and beta-xylosidases (EC 3.2.1.37). They often act in synergy with other enzymes for complete hydrolysis of hemicellulose. Xylanases find several industrial applications, for example in food and feed industries, paper and ...
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Two chitinases, designated PLC-A and PLC-B, were purified from the leayes of pokeweed (Pltytolacea americana) using DEAE-cellulose column chromatography followed by gel filtration on Sephadex G-75, hydrephobic column chromatography, and ion-exchange FPLC. PLC-A and PLC-B are acidic and basic proteins haying molecular masses of 2S and 29 kDa, and isoelectric points of 3.7 and 9.5, respectiyely. ...
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Actinomycetes were isolated from eight samples of two different habitats, i.e., garden soil and cultivated field soil. Isolation of actinomycetes was carried out on soil extract agar medium using serial dilution method and forty seven isolates were obtained. All these actinomycetes were then assessed for their antifungal activity against two fungal pathogens i.e., Candida albicans and Aspergill...
متن کاملMolecular characterization and DNA methylation profile of Libyodrilus violaceous from oil polluted soil
Studies on earthworms using molecular markers are rare in Africa except a handful from South Africa. Reports on Libyodrilus violaceous,an earthworm found in West Africaare available including their metal tolerance and bioaccumulation capacity but their molecular characterization and ecotoxicology studies are scarce. In this study, triplicate L. violaceous specimens were collec...
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ژورنال
عنوان ژورنال: African Journal of Microbiology Research
سال: 2017
ISSN: 1996-0808
DOI: 10.5897/ajmr2017.8612